Peter Connor, 31, of no fixed address, denied the charge when he appeared before Manchester Crown Court on Monday morning by video-link.
He is accused of murdering 21-year-old Billy, from Platt Bridge, on Friday, December 28.
Myles Connors, 26, of Layton Street Caravan Park, Layton Road, Preston, is also charged with murder.
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He also appeared before Judge Alan Conrad QC on Monday but has yet to enter a plea.
The pair were remanded in custody until a pre-trial review on Thursday, April 18.