His famous 2002 daubing on the wall of a Shoreditch shop, Balloon Girl, came top of a list of British art preferred by 2,000 people who were given a shortlist drawn up by arts editors and writers to choose from.
Second place went to John Constable's 1821 Romantic landscape The Hay Wain while third spot was taken by Jack Vettriano's 1992 painting The Singing Butler.
JMW Turner's 1839 work The Fighting Temeraire, also from the Romantic era, and Antony Gormley's 1998 sculpture The Angel Of The North completed the top five of a total of 20 beloved artworks.
The poll was carried out to mark the launch of a new Samsung TV, The Frame, which carries more than 100 pre-loaded artworks for users to choose from, making it into a piece of art when not being used as a television.
The full list of the nation's favourite works by British artists according to The Frame National Art Audit is as follows:
1. Banksy; Balloon Girl
2. John Constable; The Hay Wain
3. Jack Vettriano; The Singing Butler
4. JMW Turner; The Fighting Temeraire
5. Antony Gormley; The Angel of the North
6. L S Lowry; Going to the Match
7. John William Waterhouse; The Lady of Shalott
8. Peter Blake; Sgt Pepper album cover
9. Hipgnosis and George Hardie; Dark Side of the Moon album cover
10. George Stubbs; Mares and Foals
11. Thomas Gainsborough; Mr and Mrs Andrews
12. John Everett Millais; Ophelia
13. Andy Goldsworthy; Balanced Rock Misty
14. David Hockney; A Bigger Splash
15. Bridget Riley; Movement in Squares
16. Anish Kapoor; ArcelorMittal Orbit
17. Stik; A Couple Hold Hands in the Street
18. Maggi Hambling; Scallop
19. Henry Moore; Reclining Figure
20. Jamie Reid; Never Mind the Bollocks album cover