Emergency services were called to the rush hour crash in Flensburg Way, Farington Moss at 5pm.
Two of the injured were taken to Royal Preston Hospital for further treatment but their injuries were not thought to be serious, said North West Ambulance Service.
The road was reopened and rush hour traffic resumed at around 6pm after the cars were recovered.
A spokesman for the ambulance service said.“We were called at 5pm to reports of a road traffic collision involving three vehicles.
“We sent two ambulances and a rapid response vehicle.
“We took two patients to hospital but the injuries don’t seem to be serious.”