Highways were forced to close the services' M6 northbound slip road for eight hours after the spillage at 9.25pm.
A fire engine from Chorley was joined by a hazardous materials unit from Burnley as firefighters worked alongside Highways England and the Environment Agency to clean up the fuel.
Fire crews remained at the scene until 2am and used a special environmental protection kit to remove the fuel from the road surface.
A Lancashire Fire and Rescue Service spokesman said: "Working with Highways England and the Environment Agency, firefighters used an environmental protection kit with putty mats, absorbent pads and a peristaltic pump to remove the fuel.
"A fuel tank on a heavy goods vehicle had ruptured, causing spillage of a large quantity of diesel onto the Charnock Richard Services slip road."