The world number one had been waiting since a judge overturned the original decision on Monday to find out whether Immigration Minister Alex Hawke would use his powers to reimpose the penalty.
And, just before 6pm (7am UK time) on Friday, Hawke released a statement saying he had made the judgement to send Djokovic home “on health and good order grounds”.
Hawke said: “Today I exercised my power under section 133C(3) of the Migration Act to cancel the visa held by Mr Novak Djokovic on health and good order grounds, on the basis that it was in the public interest to do so.
Preston North End boss confident deals will get done as search for wing back and striker continue
Preston North End's predicted line-up for Championship clash at Luton Town
Preston North End boss provides injury update on Manchester United loanee and addresses specualtion linking them to another Red Devils youngster
Teenage Preston police cadet wins art competiton hosted by Larches and Savick Boxing Club to help keep troubled youths off the streets
Preston North End debutant talks making the step up, learning from Robbie Brady and Alvaro Fernandez and this only being the beginning
“This decision followed orders by the Federal Circuit and Family Court on 10 January 2022, quashing a prior cancellation decision on procedural fairness grounds.
“In making this decision, I carefully considered information provided to me by the Department of Home Affairs, the Australian Border Force and Mr Djokovic.
“The Morrison Government is firmly committed to protecting Australia’s borders, particularly in relation to the Covid-19 pandemic.”
Djokovic arrived in Melbourne on January 5 having been granted an exemption through Tennis Australia from the country’s strict entry rules regarding Covid-19 vaccination on the grounds that he had been recently infected with the virus.
But he was stopped by the Australian Border Force and questioned through the night before being informed that his visa had been cancelled. He was then taken to a detention hotel.
Djokovic appealed against the decision and five days later a judge ruled in his favour, seemingly freeing him up to play in the Australian Open, which begins on Monday.
There could yet be a further legal challenge from the world number one but, if not, his hopes of winning a 10th title at Melbourne Park and 21st grand slam crown are at an end.
Djokovic headed straight to Melbourne Park after being freed from the hotel on Monday and has practised every day since, including early on Friday morning, but his hopes of staying in the country have faded as the week has gone on following revelations about his conduct.
Documents revealed Djokovic tested positive in Serbia on December 16 but he was photographed at events on the following two days and issued a statement earlier this week admitting he took part in an interview with French newspaper L’Equipe at his tennis centre in Belgrade despite knowing he had the virus.
He also admitted his declaration form falsely claimed he had not travelled in the 14 days prior to his trip to Australia, which he attributed to a mistake from his agent.