The game took place at North End’s Euxton training ground and saw Robbie Brady play his first game for the club, alongside Ben Woodburn, the pair fresh from signing one-year-deals on Monday.
An 80th minute strike from Jon Nolan decided the game. PNE’s next action will come in a week’s time during their Spain summer camp as they face La Liga side Getafe.
It was the second game in three days for Ryan Lowe’s side after beating Bamber Bridge 3-0 on Saturday.
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The game was not open to the media nor fans but received some coverage on the club's social media channels.
North End had the better of the chances according to updates, with Emil Riis having a go from range and both Brady and Daniel Johnson seeing shots blocked.
Brady again having a chance just before the break but he could not find the net.
The second half provided less in the way of chances as the PNE side changed dramatically. Aaron Bennet tested the Tranmere goalkeeper but could not beat him.
After 80 minutes Rovers netted their winner, Nolan firing into the roof of the net from inside the area after receiving a cross from Ethan Bristow.
PNE Starting XI: Woodman (Cornell, 45’), Storey, Lindsay (Bauer, 45’), Hughes (Cunningham, 63’), Potts (Olosunde, 63’), Whiteman (Seary, 71’), Johnson (Mawene, 63’), Woodburn (O’Neill back on, 76’), Brady (Coulton, 45’), Riis (Amaral, 63’), O'Neill (Bennett, 45’).
Subs not used: Pradic.