The M65 was shut in both directions due to a police incident between between junction 5 (Guide Interchange) and 6 (Whitebirk Interchange).
Police, paramedics and traffic officers attended the the scene after receiving reports of a person on a bridge.
Officers held all traffic just before midday today (April 28), but the incident has now been resolved and the motorway is in the process of being reopened.
A spokesperson for Lancashire Police said: "Following our earlier tweet the M65 has now reopened.
"Many thanks for your patience."
A spokesperson for North West Ambulance service said: "We were called to this location for concern for welfare.
"We took the call at 11:50am and sent an RRV and ambulance but did not take anybody to hospital."