Both adult and child ticket prices have been raised as part of the new plans.
Daily, weekly and monthly fares also saw a hike in prices under the fare revision.
The changes came into effect for passengers today.
After a summer holiday promotion by Rotala, which owns and runs Preston Bus, the company is also introducing a £3 child EasiDay ticket which will launch on Monday, September 3.
Adult single fare prices for Preston Bus journeys has gone up by 10p to £1.80 and £2.
However, the minimum adult single fare is remaining the same at £1.50.
Meanwhile, the child single flat fare was also increased by 10p to £1.40.
EasiDay tickets for adults now cost £4, up from £3.90, while the new child fare costs £3.
EasiWeek passes for adults cost £13, up from £12.50, and child tickets now cost £10.50, up from £10.
Easi28Day passes for adults cost £45, a rise from £44, and fares for children cost £35, up from £34.
Earlier this month Preston Bus overhauled its services. One of the controversial changes announced was the plan to cut back the number 14 service.