After falling behind after 78 seconds to a header from Dover’s Steven Payne, Southport fought back with first-half goals from John Marsden and Joe Connor but had to settle for a 2-2 Conference Premier draw.
However, the 105 minutes of football played with only 10 men in Tuesday’s cup replay against Tamworth had taken its toll and the visitors dominated the second half, the equaliser coming from Shaun Francis in the 70th minute.
The Sandgrounders still managed to rise to 17th in the table and are optimistic about their chances in the first round of the FA Cup at Dagenham on Saturday with three of their injured and suspended first-teamers likely to return.
In the Conference North, AFC Fylde’s unbeaten run came to an end against a Boston United side that took advantage of some poor defending from corners, as the Pilgrims ran out 3-1 winners at the Jakeman’s Stadium.
It was the Coasters’ first defeat in 13 games as goals from Zak Mills and a Carl Piergianni double for the home side did the damage.
Fylde had been in front after 18 minutes when striker Jamie Allen tucked the ball past the onrushing Boston keeper.
Bamber Bridge went out of the FA Trophy in the first qualifying round, crashing 3-1 at Spennymoor Town.
And Clitheroe were also knocked out, beaten 2-0 at home by Workington.
In the NPL First Division North, Burscough beat Radcliffe Borough 4-0 with goals from Mark Beesley (2), Liam Caddick and William Jones.
Lancaster drew 2-2 at Ossett Albion, Kane Reece (41) and Ross Hardaker (67) scoring for the hosts, an own goal from Steven Nicholson (37) and a Jacob Davis (56) strike being the Dolly Blues’ reply.
Jordan Connerton scored four times in Kendal Town’s 6-0 win against Salford City.